https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 A stallion spermatozoon's journey through the mare's genital tract: In vivo and in vitro aspects of sperm capacitation https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50152 Wed 28 Feb 2024 10:47:42 AEDT ]]> From peptide masses to pregnancy maintenance: a comprehensive proteomic analysis of the early equine embryo secretome, blastocoel fluid, and capsule https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30545 Wed 15 Dec 2021 16:09:36 AEDT ]]> Developing a reproducible protocol for culturing functional confluent monolayers of differentiated equine oviduct epithelial cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44821 90% of the monolayers showed <1% ciliated EOECs). Occasionally (5–10%), re-differentiated monolayers with 11–27% EOECs with secondary cilia in a diffuse pattern were obtained. Additionally, nuclear progesterone receptor expression was found to be inhibited by simulated luteal phase hormone concentrations, and sperm binding to cilia was higher for re-differentiated EOEC monolayers exposed to estrogen–progesterone concentrations mimicking the follicular rather than luteal phase. Overall, a functional equine oviduct model was established with close morphological resemblance to in vivo oviduct epithelium.]]> Mon 24 Oct 2022 09:45:31 AEDT ]]> Bicarbonate-Stimulated Membrane Reorganization in Stallion Spermatozoa https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48585 0.05). After an early increase in membrane fluidity, a significant and cAMP-dependent increase in viable sperm with phosphatidylserine (PS), but not phosphatidylethanolamine (PE) exposure was noted. While the events observed partly resemble findings from the in vitro capacitation of sperm from other mammalian species, the lack of cholesterol removal appears to be an equine-specific phenomenon. This research will assist in the development of a defined medium for the capacitation of stallion sperm and will facilitate progress toward a functional IVF protocol for horse gametes.]]> Fri 21 Apr 2023 10:04:52 AEST ]]>